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The short answer is YES provided if the diaper rash is from bacterial in nature. You should consult with the doctor to see if Neosporin is the right treatment option for your baby’s diaper rash. You can put Neosporin on a diaper rash with just a small amount of it. However, you cannot overdose an antibiotic ointment like Neosporin; and if your baby’s diaper rash is bacterial, putting Neosporin can.
Some doctors recommend using a small amount of an antibiotic/antiseptic ointment like Bacitracin or Neosporin after each diaper change while the rash persists. If your baby’s rash is bacterial, this ointment can.
It looks like little open sores all over her butt. : ( The dr gave me nystatin at first and that hasn’t helped at all, in fact it’s gotten worse. Today I called back and they said to put neosporin. I googled it and the neosporin website says not to put it on a diaper rash so now I’m nervous. I’m not using.
Can I use NEOSPORIN® First Aid Antibiotics on my baby’s diaper rash? No, however, DESITIN (link is external)® diaper rash ointment is an effective diaper rash treatment. Click to see full answer. Also asked, can you use triple antibiotic ointment for diaper rash?

Can You Use Neosporin On Babies Diaper Rash
With a small amount of Neosporin, you can use it to your baby’s diaper rash once or twice a day. Make sure to clean the affected area so that it will be free from dirt before applying Neosporin. Are there any side-effects of using Neosporin?
Can You Use Too Much Neosporin?
Try to avoid using too much extra Neosporin. Using too much will oversaturate your wound and waste the Neosporin. Avoid touching the tip of the Neosporin tube with your hands, wound, or other surfaces. Put the cap back on the tube of Neosporin as soon as possible.
How Long To Use Neosporin?
How long you use the product depends on the type of wound you have. You can ask your doctor how long you should use Bacitracin or Neosporin. Do not use either product for longer than seven days unless your doctor tells you to.
Is Neosporin A Topical Antibiotic?
Neosporin ointment and cream belong to group of drugs called topical antibiotics. They kill bacteria by keeping the germs from the protein they need to build cell walls. First manufactured by Citron Pharma, The Food and Drug Administration (FDA) approved Neosporin as a prescription drug in 1971.
Is Neosporin Good For Dry Skin?
Neosporin contains antibiotics, and indiscriminate overuse of antibiotics can lead to antibiotic resistance. This means that overuse of the antibiotic may lead to infections that are resistant to treatment with antibiotics. Neosporin may help with dry skin, but only due to its inactive ingredients.